Prokhorova, AnnaAnnaProkhorovaSturm-Richter, KatrinKatrinSturm-RichterDötsch, AndreasAndreasDötschGescher, JohannesJohannesGescher2022-09-062022-09-062017-03-02Applied and Environmental Microbiology 83 (6): e03033-16 (2017)http://hdl.handle.net/11420/13559Anode-associated multispecies exoelectrogenic biofilms are essential for the function of bioelectrochemical systems (BESs). The individual activities of anodeassociated organisms and physiological responses resulting from coculturing are often hard to assess due to the high microbial diversity in these systems. Therefore, we developed a model multispecies biofilm comprising three exoelectrogenic proteobacteria, Shewanella oneidensis, Geobacter sulfurreducens, and Geobacter metallireducens, with the aim to study in detail the biofilm formation dynamics, the interactions between the organisms, and the overall activity of an exoelectrogenic biofilm as a consequence of the applied anode potential. The experiments revealed that the organisms build a stable biofilm on an electrode surface that is rather resilient to changes in the redox potential of the anode. The community operated at maximum electron transfer rates at electrode potentials that were higher than 0.04 V versus a normal hydrogen electrode. Current densities decreased gradually with lower potentials and reached half-maximal values at -0.08 V. Transcriptomic results point toward a positive interaction among the individual strains. S. oneidensis and G. sulfurreducens upregulated their central metabolisms as a response to cultivation under mixed-species conditions. G. sulfurreducens was detected in the planktonic phase of the bioelectrochemical reactors in mixed-culture experiments but not when it was grown in the absence of the other two organisms.en1098-533620176Soc.Bioelectrochemical systemsExoelectrogenic biofilmGeobacterShewanellaTranscriptome analysisBiowissenschaften, BiologieJournal Article10.1128/AEM.03033-1628087529Nojiri, HideakiHideakiNojiriOther