Ziegler, SibylleSibylleZieglerWaidner, BarbaraBarbaraWaidnerItoh, TakashiTakashiItohSchumann, PeterPeterSchumannSpring, StefanStefanSpringGescher, JohannesJohannesGescher2023-01-242023-01-242013-04-01International Journal of Systematic and Evolutionary Microbiology 63 (PART4): 1499-1504 (2013-04-01)http://hdl.handle.net/11420/14621A Gram-stain-negative, non-motile, facultatively anaerobic, acid-tolerant rod, designated strain DKE6T, was isolated from an acidic biofilm (pH 2.5) harvested in the pyrite mine Drei Kronen und Ehrt in Germany. The isolate grew optimally at pH 5.5, between 25 and 30 °C and only with casein as the carbon and energy source; although a variety of sugars were tested as growth substrates, none supported growth of the isolate. During casein consumption, strain DKE6T produced and hence modulate the pH towards the growth optimum. The predominant fatty acids (>5%) were iso-C11:0 3-OH, iso-C15:0, iso-C17:0 and iso-C17:1 ω9c. The DNA G+C content was 66.6%. Strain DKE6T was not able to oxidize iron or thiosulfate. Iron reduction was detected. The isolate showed 93.3% 16S rRNA gene sequence similarity to the most closely related cultivable strain, Dokdonella koreensis DS-123T, but <93.2% sequence similarity with other type strains of closely related type species of the Gammaproteobacteria. On the basis of physiological and biochemical data, the isolate is considered to represent a novel species of a new genus in the class Gammaproteobacteria, for which we propose the name Metallibacterium scheffleri gen. nov., sp. nov. The type strain of the type species is DKE6T (=DSM 24874T=JCM 17596T).en1466-50342013414991504Microbiology SocietyBiowissenschaften, BiologieJournal Article10.1099/ijs.0.042986-022863988Other