Chen, ZhenZhenChenGeng, FengFengGengZeng, An-PingAn-PingZeng2020-03-172020-03-172016-10-04Biotechnology Journal 10 (11): 1368-1368 (2016-10-04)http://hdl.handle.net/11420/5401The protocol for the activity array of the enzyme glutamate dehydrogenase (GDH) used in our previous publication (doi: 10.1002/biot.201400235) should be corrected as follows: Purified proteins were collected in solutions without supplementation of dithiothreitol in order to avoid its possible influence on the absorbance at 340 nm when measuring the enzymatic activity, especially when homoserine was used as the substrate. With the new protocol, the specific activity of the wild type GDH towards homoserine was determined as 0.18±0.04 mU/mg protein and the specific activity of the mutant K92V/T195S towards homoserine was 3.47±0.01 mU/mg protein. The kinetic parameters of GDH listed in Fig. 2B should also be corrected due to a mistake of units used in calculation of the Vmax.en1860-7314201613681368Wiley-VCHIngenieurwissenschaftenText10.1002/biot.201670103Other