Chatzifragkou, AfroditiAfroditiChatzifragkouAggelis, GeorgeGeorgeAggelisKomaitis, Michalis E.Michalis E.KomaitisZeng, An-PingAn-PingZengPapanikolaou, SeraphimSeraphimPapanikolaou2022-07-152022-07-152011-09-12Bioresource Technology 102 (22): 10625-10632 (2011)http://hdl.handle.net/11420/13163The impact of anaerobiosis strategy on 1,3-propanediol production during cultivation of Clostridium butyricum VPI 1718 in different size bioreactors was studied. In batch trials with N 2 gas infusion, the fermentation was successfully accomplished, regardless of initial glycerol concentration imposed and bioreactor geometry. However, in the absence of N 2 continual sparging, significant variations concerning the biochemical response of the strain were observed. Specifically, at 1-L bioreactor, the absence of N 2 infusion at high initial glycerol concentration induced lactate dehydrogenase activity and thus lactic acid synthesis, probably due to partial blockage of phosphoroclastic reaction caused by insufficient self-generated anaerobiosis environment. During fed-batch cultivation with continual N 2 sparging, the strain produced ∼71gL -1 of 1,3-propanediol, whereas under self-generated anaerobiosis, 1,3-propanediol pathway was evidently restricted, as only 30.5gL -1 of 1,3-propanediol were finally produced. Apparently, N 2 infusion strategy paired with bioreactor geometry can alter the biochemical behavior of the particular strain.en1873-29762011221062510632Elsevier Science1,3-PropanediolClostridium butyricumCrude glycerolFed-batchLactate dehydrogenaseBiowissenschaften, BiologieJournal Article10.1016/j.biortech.2011.09.02321967709Journal Article