Liese, AndreasAndreasLieseGröger, HaraldHaraldGröger2026-08-202026-08-202025-06-30Technische Universität Hamburg (2025)https://hdl.handle.net/11420/64436Threonine aldolases (LTA) have been demonstrated to be a synthetically valuable enzyme class for performing diastereoselective C-C forming reactions. Such biotransformations are of interest for the preparation of pharmaceuticals and fine chemicals as they catalyze asymmetric aldol reactions with glycine as readily available aldol donor, thus resulting in the formation of α-amino-β-hydroxycarboxylic acids. So far, a major limitation has been the non-favourable diastereoselectivity due to the switch from a kinetic to thermodynamic control of the biocatalytic reaction system. This project aimed for understanding and overcoming such process limitations.enhttps://creativecommons.org/licenses/by/4.0/Aldolaseenzymesbiotechnologyreaction engineeringprocess developmentNatural Sciences and Mathematics::572: BiochemistryTechnology::660: Chemistry; Chemical Engineering::660.6: BiotechnologyReport10.15480/882.18003