Liu, YuxinYuxinLiuWei, ZhengZhengWeiDamian, MatteoMatteoDamianZhu, XingjunXingjunZhuKnaus, TanjaTanjaKnausZhang, HongHongZhangMutti, Francesco G.Francesco G.MuttiLoeffler, Felix F.Felix F.Loeffler2025-11-262025-11-262023-11-23Advanced Materials 35 (47): 2306615 (2023)https://hdl.handle.net/11420/59158Recyclable fluorescence assays that can be stored at room temperature would greatly benefit biomedical diagnostics by bringing sustainability and cost-efficiency, especially for point-of-care serodiagnostics in developing regions. Here, a general strategy is proposed to generate recyclable fluorescent probes by using engineered enzymes with enhanced thermo-/chemo-stability, which maintains an outstanding serodiagnostic performance (accuracy >95%) after 10 times of recycling as well as after storage at elevated temperatures (37 °C for 10 days). With these three outstanding properties, recyclable fluorescent probes can be designed to detect various biomarkers of clinical importance by using different enzymes.en1521-4095202347detectionlanthanidesnanoparticlespoint-of-careTechnology::600: TechnologyJournal Article10.1002/adma.202306615Journal Article