Zhang, ChijianChijianZhangTraitrongsat, PawinPawinTraitrongsatZeng, An-PingAn-PingZeng2023-03-142023-03-142023-01-17Bioprocess and Biosystems Engineering 46 (4): 565-575 (2023-04-01)http://hdl.handle.net/11420/14993In this study, we show how electrochemically mediated bioconversion can greatly increase the co-production of 1,3-propanediol and organic acids from glycerol in an industrial bioprocess using a Clostridum pasteurianum mutant. Remarkably, an enhanced butyrate formation was observed due to a weakened butanol pathway of the mutant. This allowed the strain to have a higher ATP generation for an enhanced growth, higher glycerol consumption and PDO production. The PDO titer reached as high as 120.67 g/L at a cathodic current of -400 mA, which is 33% higher than that without electricity, with a concurrent increase of butyric acid by 80%. To fully recover the increased PDO and organic acids, a novel downstream process combining thin film evaporation of PDO and esterification of organic acids with ethanol was developed. This enables the efficient co-production of PDO, ethyl acetate and ethyl butyrate with a high overall carbon use of 87%.en1615-760520234565575Springer1,3-propanediolClostridium pasteurianumCo-productionElectro-fermentationOrganic acid estersBiowissenschaften, BiologieJournal Article10.1007/s00449-022-02841-636648555Journal Article