CHOLife: Multiscale experimental analysis and simulation of lifelines in bioreactors to study their impact on the cultivation per-formance of Chinese Hamster Ovary (CHO) cells
Chinese hamster ovary (CHO) cells have developed as the commonly applied ‘work horses’ in industrial biopharmaceutical processes for the production of proteins such as antibodies. Typical-ly, the primal tissue cells are used as suspension cultures in 12,000 – 20,000 L stirred tank bio-reactors mostly running in fed-batch mode. Gradients of dissolved gases, substrates and pH ex-ist in those settings which are similar to microbial applications and which are mainly caused by low power inputs that reduce mixing qualities. Among others, pCO2 stress is likely to effect the cellular performance under industrial conditions which may be caused by their origin as tissue cells.CHOLife tackles the scale-up problem linking the unique access to a 12,000 L cell culture biore-actor (IMS) with the development of a novel scale-up device (IBVT) finally to predict industrial scale performance of IgG1 producing CHO cells (IMS, IBVT). In essence, three dimensional flow trajectories and lifelines will be measured at IMS and mimicked in the novel scale-up simulator of IBVT. The close cooperation of both parties ensures a successful scale-down of large scale conditions and an equally successful prediction of large-scale performance of CHO cells cultured in the novel scale-up simulator.