M03.008 Determination of free carbohydrates and organic acids by ion exclusion HPLC-RI
Citation Link: https://doi.org/10.15480/336.2214
Type
Text
Date Issued
2017-02-06
Language
English
Institute
TORE-DOI
TORE-URI
Abstract
Aqueous samples are centrifuged and directly analysed by an HPLC system coupled to a refractive index detector to determine free carbohydrates such as cellobiose, glucose, xylose, arabinose and degradation products like HMF and furfural. Bound carbohydrates are extracted with acid hydrolysis (4% H2SO4) beforehand. Separation is performed with an ion exclusion HPLC phase column.
Subjects
Analytical method
Carbohydrates
DDC Class
540: Chemie
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M03.008 - Determination of carbohydrates and organic acids.pdf
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