M03.008 Determination of free carbohydrates and organic acids by ion exclusion HPLC-RI

Aqueous samples are centrifuged and directly analysed by an HPLC system coupled to a refractive index detector to determine free carbohydrates such as cellobiose, glucose, xylose, arabinose and degradation products like HMF and furfural. Bound carbohydrates are extracted with acid hydrolysis (4% H2SO4) beforehand. Separation is performed with an ion exclusion HPLC phase column.