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  4. Enrichment of phosphate-accumulating organisms (PAOs) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime
 
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Enrichment of phosphate-accumulating organisms (PAOs) in a microfluidic model biofilm system by mimicking a typical aerobic granular sludge feast/famine regime

Citation Link: https://doi.org/10.15480/882.4163
Publikationstyp
Journal Article
Date Issued
2022-01-15
Sprache
English
Author(s)
Klein, Edina  
Weiler, Janek  
Wagner, Michael  
Čelikić, Minja  
Niemeyer, Christof M.  
Horn, Harald  
Gescher, Johannes  
Institut
Technische Mikrobiologie V-7  
TORE-DOI
10.15480/882.4163
TORE-URI
http://hdl.handle.net/11420/11718
Journal
Applied microbiology and biotechnology  
Volume
106
Issue
3
Start Page
1313
End Page
1324
Citation
Applied Microbiology and Biotechnology 106 (3): 1313-1324 (2022-02-01)
Publisher DOI
10.1007/s00253-022-11759-8
Scopus ID
2-s2.0-85123114514
PubMed ID
35032186
Publisher
Springer
Wastewater treatment using aerobic granular sludge has gained increasing interest due to its advantages compared to conventional activated sludge. The technology allows simultaneous removal of organic carbon, nitrogen, and phosphorus in a single reactor system and is independent of space-intensive settling tanks. However, due to the microscale, an analysis of processes and microbial population along the radius of granules is challenging. Here, we introduce a model system for aerobic granular sludge on a small scale by using a machine-assisted microfluidic cultivation platform. With an implemented logic module that controls solenoid valves, we realized alternating oxic hunger and anoxic feeding phases for the biofilms growing within. Sampling during ongoing anoxic cultivation directly from the cultivation channel was achieved with a robotic sampling device. Analysis of the biofilms was conducted using optical coherence tomography, fluorescence in situ hybridization, and amplicon sequencing. Using this setup, it was possible to significantly enrich the percentage of polyphosphate-accumulating organisms (PAO) belonging to the family Rhodocyclaceae in the community compared to the starting inoculum. With the aid of this miniature model system, it is now possible to investigate the influence of a multitude of process parameters in a highly parallel way to understand and efficiently optimize aerobic granular sludge-based wastewater treatment systems. Key points • Development of a microfluidic model to study EBPR. • Feast-famine regime enriches polyphosphate-accumulating organisms (PAOs). • Microfluidics replace sequencing batch reactors for aerobic granular sludge research.
Subjects
Aerobic granular sludge (AGS)
Biofilms
Enhanced biological phosphorus removal (EBPR)
Microfluidic
Polyphosphate-accumulating organisms (PAOs)
Wastewater treatment
DDC Class
600: Technik
More Funding Information
We gratefully acknowledge financial support through the Helmholtz programmes “BioInterfaces in Technology and Medicine” and “Adaptive and Bioinstructive Materials Systems.”. Open Access funding enabled and organized by Projekt DEAL.
Publication version
publishedVersion
Lizenz
https://creativecommons.org/licenses/by/4.0/
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