Debottlenecking a 2-phase multi-enzymatic cascade by an enzyme membrane reactor – Modelling and experimental validation

Biotechnological processes have a high potential to make industrial processes more sustainable. However, biotechnological processes often have low product concentrations and production rates. This is where process intensification can help to make these processes competitive. Using the example of the multi-enzymatic synthesis of natural cinnamyl cinnamate, it is shown how an existing multi-enzymatic process can be intensified by using an enzyme membrane reactor. The individual enzymatic reactions are first characterised in laboratory scale and then combined in a mini plant. In addition, a process model of the mini plant is developed. It is shown that the use of an enzyme membrane reactor can increase the production rate of the multi-enzymatic process by a factor of 10 compared to a previously investigated set-up.

Subjects

Alcohol dehydrogenase

Enzyme membrane reactor

Formate dehydrogenase

Polymeric membranes

Ultrafiltration

DDC Class

570: Life Sciences, Biology

660: Chemistry; Chemical Engineering

Funding(s)

Projekt DEAL

Publication version

publishedVersion

Lizenz

https://creativecommons.org/licenses/by/4.0/

Name

1-s2.0-S0255270125003459-main.pdf

Size

2.31 MB

Format

Adobe PDF

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Debottlenecking a 2-phase multi-enzymatic cascade by an enzyme membrane reactor – Modelling and experimental validation