Implementation of mDoE-methods to a microcarrier-based expansion processes for mesenchymal stem cells

The need for advanced therapy medicinal products (ATMPs) has gained increased attention in recent years. In this respect, a well-designed cell expansion process is needed to efficiently manufacture the required number of cells with the desired product quality. This step is challenging due to the biological complexity of the respective primary cell (e.g., mesenchymal stem cells (MSC)) and the usage of microcarrier-based expansion systems. One accelerating approach for process design is model-assisted Design of Experiments (mDoE) combining mathematical process models and statistical tools. In this study, the mDoE workflow was used for the development of an expansion processes with human immortalized mesenchymal stem cells (hMSC-TERT) and the aim of maximizing cell yield assuming only a limited amount of prior knowledge at a very early stage of development. First, suitable microcarriers for expansion in shake flasks were screened and the differentiation of the cells was proven. Second, initial experiments were performed to generate prior knowledge, which was then used to set up the mathematical model and to estimate the model parameters. Finally, the mDoE was used to determine and evaluate the design space to be performed experimentally. Overall, a cell expansion process using microcarriers in a shake flask culture was successfully implemented and a significant increase in cell yield (up to 6,2-fold) was achieved compared to literature.

Subjects

bioprocess modeling

early stage development

hMSC-TERT

microcarrier culture

model-assisted Design of Experiments

process design

DDC Class

660.6: Biotechnology

Funding(s)

Projekt DEAL

Publication version

publishedVersion

Lizenz

https://creativecommons.org/licenses/by/4.0/

Name

Biotechnology Progress - 2024 - Kuchemüller - Implementation of mDoE‐methods to a microcarrier‐based expansion processes.pdf

Type

Main Article

Size

2.03 MB

Format

Adobe PDF

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Implementation of mDoE-methods to a microcarrier-based expansion processes for mesenchymal stem cells