Enhanced CO₂ fixation in the biocatalytic carboxylation of resorcinol: utilization of amines for amine scrubbing and in situ product precipitation

Publikationstyp
Journal Article
Date Issued
2021-02
Sprache
English
Author(s)
Ohde, Daniel  orcid-logo
Thomas, Benjamin  
Bubenheim, Paul  orcid-logo
Liese, Andreas  orcid-logo
Institut
Technische Biokatalyse V-6  
TORE-URI
http://hdl.handle.net/11420/7900
Journal
Biochemical engineering journal  
Volume
166
Article Number
107825
Citation
Biochemical Engineering Journal (166): 107825 (2021-02)
Publisher DOI
10.1016/j.bej.2020.107825
Scopus ID
2-s2.0-85096199749
Reversible enzymatic decarboxylation reactions are known for their thermodynamic limitations regarding the carboxylation. Significant thermodynamic and kinetic improvements were achieved for the carboxylation of resorcinol by the 2,6-dihydroxybenzoic acid decarboxylase from Rhizobium sp. utilizing the amine scrubber triethanolamine for CO₂ mediation. In a first approach, maximal yields of up to 50 % are achieved and are found to correlate with the CO₂ loading capacity of triethanolamine. In a second approach, application of quaternary amines for in situ carboxylation product removal by precipitation improves the achievable yields depending on utilized amine concentration. This demonstrates the possibility for a reusable system. By variation of hydrated CO₂ species ratio and concentration, CO₂ and alternatively H₂CO₃ is identified acting as second substrate.
Subjects
Biotransformation
Carboxylation
CO fixation 2
Decarboxylase
Reaction mechanism
DDC Class
600: Technik