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  4. Successful bi-enzyme stabilization for the biomimetic cascade transformation of carbon dioxide
 
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Successful bi-enzyme stabilization for the biomimetic cascade transformation of carbon dioxide

Publikationstyp
Journal Article
Date Issued
2016-07-20
Sprache
English
Author(s)
Hwang, Ee Taek  
Seo, Bo-Kuk  
Gu, Man Bock  
Zeng, An-Ping  orcid-logo
Institut
Bioprozess- und Biosystemtechnik V-1  
TORE-URI
http://hdl.handle.net/11420/5456
Journal
Catalysis science & technology  
Volume
6
Issue
19
Start Page
7267
End Page
7272
Citation
Catalysis Science and Technology 19 (6): 7267-7272 (2016)
Publisher DOI
10.1039/c6cy00783j
Scopus ID
2-s2.0-84989282203
Publisher
RSC Publ.
In nature, carbon dioxide (CO2) conversion to valuable chemicals occurs via several metabolic pathways through multi-enzymatic reactions. Here, we aimed to mimic this by introducing enzyme immobilization in microbead compartments forming a stabilized multi-enzyme system. The system is assembled by encapsulation of phosphoenolpyruvate carboxylase (PEPCase) in branched polymeric microbeads followed by carbonic anhydrase (CA) immobilization on the silica-shell surface of the microbeads. The step-by-step construction of the CA/PEPCase microbeads is monitored based on the stability of each enzyme and cascade enzymatic oxaloacetate (OAA) production rate from a CO2 substrate. Each CA and PEPCase in the microbeads preserved their catalytic activity even after 20 times of reuse, with facile magnetic separability at room temperature. The CA/PEPCase system retained about 75% of the OAA production rate of free CA/PEPCase by forming a multi-enzyme/microbead complex structure. To the best of our knowledge, this report is the first demonstration of a stabilized cascade CA/PEPCase system that mimics the biomimetic CO2 conversion by a multi-enzymatic pathway found in biological systems.
DDC Class
570: Biowissenschaften, Biologie
600: Technik
More Funding Information
Supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (NRF- 1ABA001-2010-0020501), in part by the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning
(2015M1A5A1037055), and in part by the German Federal Ministry for Research and Education through the project
Komparti (FKZ 031A174).
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