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Automatisierte Charakterisierung und Optimierung elektroaktiver Biofilme
Citation Link: https://doi.org/10.15480/882.13235
Publikationstyp
Doctoral Thesis
Date Issued
2024
Sprache
German
Author(s)
Advisor
Referee
Title Granting Institution
Technische Universität Hamburg
Place of Title Granting Institution
Hamburg
Examination Date
2024-05-15
Institute
TORE-DOI
Citation
Technische Universität Hamburg (2024)
Microbial electrochemical systems (MES) represent a highly versatile platform technology with an immense potential for the development towards a sustainable bioeconomy, whereby the industrial realization of this potential requires a critical focus on biofilm optimization.
In order to be able to directly correlate biofilm development and current production as well as spatially resolve biofilm activity, a microfluidic cultivation platform with integrated automated three-dimensional imaging was established.
The platform was validated by investigating a model community of G. sulfurreducens and S. oneidensis, revealing a co-dependency in which G. sulfurreducens relies on the nutrient supply from S. oneidensis, while S. oneidensis in turn utilizes the conductive biofilm matrix developed by G. sulfurreducens, facilitating direct interspecies electron transfer (DIET) to the anode.
Moreover, the influence of different genetic factors on the biofilm development of \textit{S.~oneidensis} was fundamentally investigated.
The host strain used for these studies was S. oneidensis MR-1 Δλ, based on the hypothesis that the Δλ-induced reduction of extracellular polymeric substances (EPS) significantly facilitates electron transport through the biofilm.
By investigating various genetic factors, it could be shown that under oxic conditions, superordinate mechanisms seem to take effect and determine biofilm development, whereas under anoxic conditions with fumarate as electron acceptor, both qualitative and quantitative differences compared to the control strains could be determined.
Using an anode as the terminal electron acceptor, co-overexpression of wbpA and wbpP, overexpression of bpfA, a point-mutated bpfG variant and the simple addition of an empty plasmid vector led to a 1.67 - 1.85-fold increase in mean current density.
In summary, this work identified and characterized key factors of anaerobic biofilm formation, providing a basis for the cultivation of biofilms with optimized user-defined properties.
In order to be able to directly correlate biofilm development and current production as well as spatially resolve biofilm activity, a microfluidic cultivation platform with integrated automated three-dimensional imaging was established.
The platform was validated by investigating a model community of G. sulfurreducens and S. oneidensis, revealing a co-dependency in which G. sulfurreducens relies on the nutrient supply from S. oneidensis, while S. oneidensis in turn utilizes the conductive biofilm matrix developed by G. sulfurreducens, facilitating direct interspecies electron transfer (DIET) to the anode.
Moreover, the influence of different genetic factors on the biofilm development of \textit{S.~oneidensis} was fundamentally investigated.
The host strain used for these studies was S. oneidensis MR-1 Δλ, based on the hypothesis that the Δλ-induced reduction of extracellular polymeric substances (EPS) significantly facilitates electron transport through the biofilm.
By investigating various genetic factors, it could be shown that under oxic conditions, superordinate mechanisms seem to take effect and determine biofilm development, whereas under anoxic conditions with fumarate as electron acceptor, both qualitative and quantitative differences compared to the control strains could be determined.
Using an anode as the terminal electron acceptor, co-overexpression of wbpA and wbpP, overexpression of bpfA, a point-mutated bpfG variant and the simple addition of an empty plasmid vector led to a 1.67 - 1.85-fold increase in mean current density.
In summary, this work identified and characterized key factors of anaerobic biofilm formation, providing a basis for the cultivation of biofilms with optimized user-defined properties.
Subjects
Bioelectrochemical system
Biofilm
Microbial electrolysis cell
Microfluidics
DDC Class
620.11: Engineering Materials
621.3: Electrical Engineering, Electronic Engineering
570: Life Sciences, Biology
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