Experimental studies on microscopic, multiphase transport phenomena: novel applications of light sheet fluorescence microscopy

Over the past two decades, light sheet fluorescence microscopy has developed to a powerful tool for studies of dynamics in biological systems. In a new development, we apply light sheet fluorescence microscopy as a novel experimental measurement technology within the field of multiphase process engineering and fluid dynamics. The technology enables a number of novel studies of single- and multiphase transport phenomena on a microscopic scale. In the current publication, we introduce the first implementation of light sheet fluorescence microscopy in the field and demonstrate its applicability on one exemplary measurement of diffusive oxygen mass transfer from an oxygen bubble to degassed water. The results prove that such measurements can be conducted with a high spatial resolution with a submicron pixel pitch and enable precise studies on microscopic transport phenomena. Besides measurements of mass transfer, on which we lay the focus here, light sheet fluorescence microscopy further enables studies of fluid dynamics on a microscopic scale.

Subjects

LSFM

Mass transfer

PLIF

SPIM

DDC Class

660: Chemistry; Chemical Engineering

Funding(s)

Feinblasen für biokatalytische Prozesse: Mikroskalige Phänomene und neuartige Anwendungen

Projekt DEAL

Lizenz

https://creativecommons.org/licenses/by/4.0/

Publication version

publishedVersion

Name

s12650-026-01115-7.pdf

Type

Main Article

Size

5.42 MB

Format

Adobe PDF

Options

Experimental studies on microscopic, multiphase transport phenomena: novel applications of light sheet fluorescence microscopy