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Combinatorial Synthesis of Macromolecular Arrays by Microchannel Cantilever Spotting (µCS)
Publikationstyp
Journal Article
Date Issued
2018-08-02
Sprache
English
Author(s)
Atwater, Jordyn
Streit, Bettina
Fuchs, Harald
Hirtz, Michael
Journal
Volume
30
Issue
31
Article Number
1801632
Citation
Advanced Materials 30 (31): 1801632 (2018)
Publisher DOI
Scopus ID
ISSN
09359648
Surface-bound microarrays of multiple oligo- and macromolecules (e.g., peptides, DNA) offer versatile options in biomedical applications like drug screening, DNA analysis, or medical diagnostics. Combinatorial syntheses of these molecules in situ can save significant resources in regard to processing time and material use. Furthermore, high feature densities are needed to enable high-throughput and low sample volumes as generally regarded in combinatorial chemistry. Here, a scanning-probe-lithography-based approach for the combinatorial in situ synthesis of macromolecules is presented in microarray format. Feature sizes below 40 µm allow for the creation of high-density arrays with feature densities of 62 500 features per cm<sup>2</sup>. To demonstrate feasibility of this approach for biomedical applications, a multiplexed array of functional protein tags (HA- and FLAG-tag) is synthesized, and selective binding of respective epitope recognizing antibodies is shown. This approach uses only small amounts of base chemicals for synthesis and can be further parallelized, therefore, opening up a route to flexible, highly dense, and cost-effective microarrays.
Subjects
combinatorial chemistry
high-throughput screening
microarrays
peptides
scanning probe lithography
DDC Class
600: Technology