Biochemical synthesis: Combinatorial chemistry in high-density array format

Publikationstyp
Journal Article
Date Issued
2016-09-01
Sprache
English
Author(s)
Loeffler, Felix F.  
Breitling, Frank  
Nesterov-Müller, Alexander
TORE-URI
https://hdl.handle.net/11420/61623
Journal
Biospektrum  
Volume
22
Issue
5
Start Page
532
End Page
534
Citation
Biospektrum 22 (5): 532-534 (2016)
Publisher DOI
10.1007/s12268-016-0720-1
Scopus ID
2-s2.0-84987597642
ISSN
09470867
We synthesize peptide arrays by using laser pulses to transfer amino acids onto a synthesis slide that are embedded in a polymer. After patterning these razor-thin spots, the coupling starts by heating the polymer that now serves as a solvent to couple monomers to the surface. The main advantages of this nanolayer solid phase chemistry are the high degree of automation, the very high spot density, and the in situ activation of monomers due to mixing different reagents in the stacked nanolayers.
DDC Class
600: Technology