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Optimization of hydrolysis conditions for xylans and straw hydrolysates by HPLC analysis
Citation Link: https://doi.org/10.15480/882.4853
Publikationstyp
Journal Article
Publikationsdatum
2023-02
Sprache
English
Author
Enthalten in
Volume
13
Start Page
3361
End Page
3374
Citation
Biomass Conversion and Biorefinery (13): 3361–3374 (2023-02)
Publisher DOI
Scopus ID
Publisher
Springer
Oligosaccharide analysis is commonly done by acid hydrolysis and following HPLC analysis. A major problem is the incomplete hydrolysis of oligosaccharides and disaccharides and the increasing formation of volatile furfural from pentose monomers and hydroxymethylfurfural (HMF) from hexose monomers. This paper optimizes the conditions of hydrolysis approaches and proposes a method for oligosaccharide quantification. The optimal condition for hydrolysis of model xylan from corn cob was found to be for 100 °C hydrolysis temperature, 120 min hydrolysis time, and 2 wt% sulfuric acid concentration. Under these conditions, the total free and bound xylose yield was 77.4% and hemicellulose conversion 87.4% respectively; no degradation products were found. The optimal conditions for hydrolysis of model xylan from beech wood were found to be for 120 °C hydrolysis temperature, 120 min hydrolysis time, and 2 wt% sulfuric acid concentration. Under these conditions, the total free and bound xylose yield was 65.1% and hemicellulose conversion 70.5% respectively; no degradation products were found. For pentosan hydrolysate, conditions were further optimized (110 °C, 60 min, 2 wt% H2SO4). Standard addition of xylan from the corn cob for hydrolysation showed similar conversion rates (< 2% deviation); no matrix effects were detected.
Schlagworte
HPLC
Oligosaccharides
Pentosans
Quantification
Xylose
DDC Class
570: Biowissenschaften, Biologie
Projekt(e)
More Funding Information
The ELBE-NH research project was supported via t Project Management Jülich, commissioned by the FederalMinistry of Education and Research.
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