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Electrical stimulation of NIH-3T3 cells with platinum-PEDOT-electrodes integrated in a bioreactor
Citation Link: https://doi.org/10.15480/882.2186
Publikationstyp
Journal Article
Date Issued
2013
Sprache
English
Author(s)
TORE-DOI
TORE-URI
Volume
7
Issue
1
Start Page
125
End Page
132
Citation
The open biomedical engineering journal 1 (7): 125-132 (2013)
Publisher DOI
Scopus ID
Publisher
Bentham Open
The objective of this work involves the development and integration of electrodes for the electrical stimulation of cells within a bioreactor. Electrodes need to fit properties such as biocompatibility, large reversible charge transfer and high flexibility in view of their future application as implants on the tympanic membrane. Flexible thin-film platinum-poly(3,4-ethylene-dioxythiophene)-electrodes on a poly(ethylene terephthalate)-foil manufactured using microsystems technology were integrated into a bioreactor based on the design of a 24 well plate. The murine fibroblast cell line NIH-3T3 was cultured on the foil electrodes and the cells were stimulated with direct voltage and unipolar pulsed voltage. The amplitude, the pulse length and the ratio of pulse to pause were varied. The stimulated cells were stained in order to determine the angle between the cell cleavage plane of the dividing cells and the vector of the electric field. These angles were subsequently used to calculate the polarization index, which is a measure of the orientation of the metaphase plane of dividing cells that occurs for example during wound healing or embryonic morphogenesis.
Subjects
electrical stimulation
cell culture
fibroblasts
integrated biocompatible electrodes
orientation of metaphase plate
polarization index.
DDC Class
570: Biowissenschaften, Biologie
600: Technik
610: Medizin
620: Ingenieurwissenschaften
More Funding Information
Bundesministerium für Bildung und Forschung (BMBF).
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Name
TOBEJ-7-125.pdf
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1.15 MB
Format
Adobe PDF