A modular framework for post-processing and analysis of fluorescence microscopy image sequences of subcellular calcium dynamics

Start Page
401
End Page
406
Citation
Workshops on Image Processing for Medicine, 2015: 401-406
Contribution to Conference
Workshops on Image Processing for Medicine, 2015
Publisher DOI
10.1007/978-3-662-46224-9_69
Scopus ID
2-s2.0-85012215400
Publisher
Springer
ISBN
978-3-662-46224-9
978-3-662-46223-2
ISBN of container
978-3-662-46224-9
978-3-662-46223-2
Calcium (Ca2+) signaling is essential for activation of Tlymphocytes and can be understood as fundamental on-switch for the adaptive immune system. The activation is supposed to start by initial spatially and temporally localized Ca2+ signals. Imaging and analysis of these signals require high spatio-temporal resolution fluorescence microscopy – which, in turn, results in the need for an efficient and reliable post-processing and analysis workflow of the acquired image data. Started with a well established but time-consuming post-processing process, we report on our efforts to automatize and optimize it. The efforts led to a modular post-processing and analysis framework, which is presented. In addition, the influence of instances of the main blocks of the framework (e.g. bleaching correction, deconvolution) on Ca2+ dynamics analysis measures is evaluated.
DDC Class
004: Informatik
610: Medizin
More Funding Information
Supported by the Forschungszentrum Medizintechnik Hamburg (to IMAW, AS and RW) and the Deutsche Forschungsgemeinschaft (grant no GU 360/15-1 to AHG).