Protein recovery from bioethanol stillage by liquid hot water treatment

Publikationstyp
Journal Article
Date Issued
2020-01
Sprache
English
Author(s)
Lamp, Anne 
Kaltschmitt, Martin  
Lüdtke, Oliver  
Institut
Umwelttechnik und Energiewirtschaft V-9  
TORE-URI
http://hdl.handle.net/11420/3610
Journal
The journal of supercritical fluids  
Volume
155
Article Number
104624
Citation
Journal of Supercritical Fluids (155): 104624 (2020-01)
Publisher DOI
10.1016/j.supflu.2019.104624
Scopus ID
2-s2.0-85072858526
Protein recovery from biogenic residues offers a high potential for increasing the added value. This paper investigates the protein recovery from bioethanol stillage by liquid hot water treatment at pH 3–11 and 110–210 °C for 10–90 min. The results show that protein solubilization starts at 89 °C and is limited by temperature. However, all amino acids except glutamic acid start to degrade at 170 °C. Especially lysine, arginine and asparagine undergo Maillard reactions, whereby degradation is favoured by bases; acids show a stabilizing effect. The highest protein yield in the hydrolysate is 75% with a concentration of 53%-DM at 170 °C and 20 min. In addition, kinetic modelling of the rate constants for protein solubilization and amino acid degradation is performed. Rate constants for amino acid degradation are about one order of magnitude higher for polar than for non-polar amino acids.
Subjects
Bioethanol stillage
Kinetic modelling
Liquid hot water
Maillard reactions
Protein recovery
Subcritical water