Characterization data for the establishment of scale-up and process transfer strategies between stainless steel and single-use bioreactors

The reliable transfer of bioprocesses from single-use bioreactors (SUBs) of different scales to conventional stainless steel stirred-tank bioreactors is of steadily growing interest. In this publication, a scale-up study for SUBs with volumes of 200 L and 2000 L and the transfer to an industrial-scale conventional stainless steel stirred-tank bioreactor with a volume of 15,000 L is presented. The scale-up and transfer are based on a comparison of mixing times and the modeling of volumetric mass transfer coefficients kLa, measured in all three reactors in aqueous PBS/Kolliphor solution. The mass transfer coefficients are compared with the widely used correlation of van’t Riet at constant stirrer tip speeds. It can be shown that a van’t Riet correlation enables a robust and reliable prediction of mass transfer coefficients on each scale for a wide range of stirrer tip speeds and aeration rates. The process transfer from single-use bioreactors to conventional stainless steel stirred-tank bioreactors is proven to be uncritical concerning mass transfer performance. This provides higher flexibility with respect to bioreactor equipment considered for specific processes.

Subjects

correlation

kLa

Kolliphor

mass transfer

mixing time

PBS

scale up

single-use bioreactor (SUB)

stirred-tank reactor (STR)

DDC Class

660: Chemistry; Chemical Engineering

620: Engineering

Funding Organisations

Boehringer Ingelheim Pharma GmbH & Co. KG

Sartorius Stedim Biotech

Publication version

publishedVersion

Lizenz

https://creativecommons.org/licenses/by/4.0/

Name

fluids-09-00115.pdf

Type

Main Article

Size

3.92 MB

Format

Adobe PDF

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Characterization data for the establishment of scale-up and process transfer strategies between stainless steel and single-use bioreactors