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Development of genome editing method for metabolic engineering of Clostridium pasteurianum reveals new features of metabolism
Citation Link: https://doi.org/10.15480/882.13897
Publikationstyp
Doctoral Thesis
Date Issued
2024
Sprache
English
Author(s)
Advisor
Referee
Title Granting Institution
Technische Universität Hamburg
Place of Title Granting Institution
Hamburg
Examination Date
2024-11-27
Institute
TORE-DOI
Citation
Technische Universität Hamburg (2024)
Peer Reviewed
true
Clostridium pasteurianum is an industrial microorganism for production of 1,3-propanediol (PDO) and butanol, but suffers from lacking genetic tools for metabolic engineering and previous studies merely focused on single genomic modification. To this end, a new approach for consecutive genome engineering is presented for the first time based on an endogenous CRISPR-Cas method. Examination of the phenotype of generated mutants led to discovery of unexpected metabolites showing that genetic engineering can have significant impact on metabolism. The developed procedure expands the genetic toolkit for C. pasteurianum and provides researches an additional method which contributes to improved genetic accessibility of this strain.
Subjects
Clostridium pasteurianum | consecutive genome engineering | CRISPR-Cas | metabolic engineering | substrate utilization
DDC Class
660.6: Biotechnology
Funding Organisations
Hua An Tang Biotech Group Co., Ltd.
Publication version
acceptedVersion
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