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Metabolic engineering of Escherichia coli for production of mixed-acid fermentation end products
Publikationstyp
Journal Article
Date Issued
2014-05
Sprache
English
Author(s)
Gescher, Johannes
Volume
2
Issue
5
Article Number
16
Citation
Frontiers in Bioengineering and Biotechnology 2 (5): 16 (2014-05)
Publisher DOI
Scopus ID
Publisher
Frontiers Media
Mixed-acid fermentation end products have numerous applications in biotechnology. This is probably the main driving force for the development of multiple strains that are supposed to produce individual end products with high yields. The process of engineering Escherichia coli strains for applied production of ethanol, lactate, succinate, or acetate was initiated several decades ago and is still ongoing. This review follows the path of strain development from the general characteristics of aerobic versus anaerobic metabolism over the regulatory machinery that enables the different metabolic routes. Thereafter, major improvements for broadening the substrate spectrum of E. coli toward cheap carbon sources like molasses or lignocellulose are highlighted before major routes of strain development for the production of ethanol, acetate, lactate, and succinate are presented.
Subjects
Acetate
Carbon source
E. coli
Ethanol
Lactate
Mixed-acid fermentation
Succinate
DDC Class
570: Biowissenschaften, Biologie
600: Technik