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Model-based strategy for cell culture seed train layout verified at lab scale
Publikationstyp
Journal Article
Date Issued
2015-03-21
Sprache
English
Author(s)
Institut
TORE-URI
Journal
Volume
68
Issue
4
Start Page
1019
End Page
1032
Citation
Cytotechnology 4 (68): 1019-1032 (2016-08-01)
Publisher DOI
Scopus ID
Publisher
Springer Science + Business Media B.V.
Cell culture seed trains—the generation of a sufficient viable cell number for the inoculation of the production scale bioreactor, starting from incubator scale—are time- and cost-intensive. Accordingly, a seed train offers potential for optimization regarding its layout and the corresponding proceedings. A tool has been developed to determine the optimal points in time for cell passaging from one scale into the next and it has been applied to two different cell lines at lab scale, AGE1.HNAAT and CHO-K1. For evaluation, experimental seed train realization has been evaluated in comparison to its layout. In case of the AGE1.HNAAT cell line, the results have also been compared to the formerly manually designed seed train. The tool provides the same seed train layout based on the data of only two batches.
Subjects
Design
Layout
Modeling
Optimization
Seed train
Space–Time–Yield
Suspension cell cultures
DDC Class
570: Biowissenschaften, Biologie
600: Technik