Lipase-catalyzed synthesis of glucose-6-O-hexanoate in deep eutectic solvents
Enzymatic synthesis of sugar fatty acid esters in organic solvents is a well-described procedure to synthesize glycolipids. This study aims at replacing these solvents with deep eutectic solvents (DES), a group of solvents that gained more and more interest during the last years, since they can be easily produced from non-toxic resources. Enzymatic glycolipid synthesis in deep eutectic solvents was investigated, employing Candida antarctica lipase B (Novozyme 435) in various deep eutectic solvents. A successful lipase-catalyzed synthesis of glucose fatty acid esters gave proof of this concept, while using the two deep eutectic solvents consisting of choline chloride and urea (CC : U) and choline chloride and glucose (CC : Glc). Additionally the DES consisting of choline chloride and glucose was observed to act as solvent and substrate for the synthesis at the same time.
Deep eutectic solvents
More Funding Information
Funding from the partly BMBF funded ERA-IB BioSurf project (617 40003 0315928B) was gratefully acknowledged. B.L. thanks the DFG (LU 835/6-2 and instrumental facility Pro2NMR) and the Helmholtz association (programme Biointerfaces) for funding.