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Influence of varying pre-culture conditions on the level of population heterogeneity in batch cultures with an escherichia coli triple reporter strain
Citation Link: https://doi.org/10.15480/882.8810
Publikationstyp
Journal Article
Date Issued
2023-07
Sprache
English
Author(s)
Biochemical Engineering, TUM School of Engineering and Design, Technical University of Munich
Biochemical Engineering, TUM School of Engineering and Design, Technical University of Munich
Biochemical Engineering, TUM School of Engineering and Design, Technical University of Munich
Biochemical Engineering, TUM School of Engineering and Design, Technical University of Munich
Biochemical Engineering, TUM School of Engineering and Design, Technical University of Munich
TORE-DOI
Journal
Volume
11
Issue
7
Article Number
1763
Citation
Microorganisms 11 (7): 1763 (2023-07)
Publisher DOI
Scopus ID
Publisher
MDPI
Peer Reviewed
true
When targeting robust, high-yielding bioprocesses, phenomena such as population heterogeneity have to be considered. Therefore, the influence of the conditions which the cells experience prior to the main culture should also be evaluated. Here, the influence of a pre-culture medium (complex vs. minimal medium), optical density for inoculation of the main culture (0.005, 0.02 and 0.0125) and harvest time points of the pre-culture in exponential growth phase (early, mid and late) on the level of population heterogeneity in batch cultures of the Escherichia coli triple reporter strain G7BL21(DE3) in stirred-tank bioreactors was studied. This strain allows monitoring the growth (rrnB-EmGFP), general stress response (rpoS-mStrawberry) and oxygen limitation (nar-TagRFP657) of single cells through the expression of fluorescent proteins. Data from batch cultivations with varying pre-culture conditions were analysed with principal component analysis. According to fluorescence data, the pre-culture medium had the largest impact on population heterogeneities during the bioprocess. While a minimal medium as a pre-culture medium elevated the differences in cellular growth behaviour in the subsequent batch process, a complex medium increased the general stress response and led to a higher population heterogeneity. The latter was promoted by an early harvest of the cells with low inoculation density. Seemingly, nar-operon expression acted independently of the pre-culture conditions.
Subjects
batch culture
population heterogeneity
pre-culture
principal component analysis
triple reporter strain
DDC Class
610: Medicine, Health
Publication version
publishedVersion
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Name
heins_2_microorganisms-11-01763.pdf
Type
Main Article
Size
4.36 MB
Format
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