Process design of a continuous biotransformation with in situ product removal by cloud point extraction

Publikationstyp
Journal Article
Publikationsdatum
2021-05
Sprache
English
Author
Fellechner, Oliver 
Smirnova, Irina orcid-logo
Institut
Thermische Verfahrenstechnik V-8 
TORE-URI
http://hdl.handle.net/11420/8602
Enthalten in
The Canadian journal of chemical engineering 
Volume
99
Issue
5
Start Page
1035
End Page
1049
Citation
The Canadian Journal of Chemical Engineering 99 (5): 1035-1049 (2021-05)
Publisher DOI
10.1002/cjce.23967
Scopus ID
2-s2.0-85101687688
Publisher
Soc.
In this work, a continuous, heterogeneous extractive biocatalysis was realized with penicillin G hydrolysis as a model reaction. Therefore, commercially available structured packing CY from Sulzer were coated with enzyme‐containing gels and their catalytic activity was examined. Thereby, the potential of aqueous micellar two‐phase systems (ATPMS) as an extraction medium was evaluated. Triton X‐114 was used as model surfactant. It was found that the separation efficiency of the packing is not affected by the coating and ATPMS allows for a good separation yield. With these results, different process concepts for the continuous, heterogeneous extractive biocatalysis were compared.
DDC Class
600: Technik