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  4. Metagenomic potential for and diversity of N-cycle driving microorganisms in the Bothnian Sea sediment
 
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Metagenomic potential for and diversity of N-cycle driving microorganisms in the Bothnian Sea sediment

Citation Link: https://doi.org/10.15480/882.1962
Publikationstyp
Journal Article
Date Issued
2017-05-23
Sprache
English
Author(s)
Rasigraf, Olivia  
Schmitt, Julia 
Jetten, Mike S. M.  
Lüke, Claudia  
Institut
Wasserressourcen und Wasserversorgung B-11  
TORE-DOI
10.15480/882.1962
TORE-URI
https://tubdok.tub.tuhh.de/handle/11420/1965
Journal
MicrobiologyOpen  
Volume
6
Issue
4
Start Page
e00475
Citation
MicrobiologyOpen 4 (6): e00475- (2017)
Publisher DOI
10.1002/mbo3.475
Scopus ID
2-s2.0-85019623925
Publisher
Wiley
The biological nitrogen cycle is driven by a plethora of reactions transforming nitrogen compounds between various redox states. Here, we investigated the metagenomic potential for nitrogen cycle of the in situ microbial community in an oligotrophic, brackish environment of the Bothnian Sea sediment. Total DNA from three sediment depths was isolated and sequenced. The characterization of the total community was performed based on 16S rRNA gene inventory using SILVA database as reference. The diversity of diagnostic functional genes coding for nitrate reductases (napA;narG), nitrite:nitrate oxidoreductase (nxrA), nitrite reductases (nirK;nirS;nrfA), nitric oxide reductase (nor), nitrous oxide reductase (nosZ), hydrazine synthase (hzsA), ammonia monooxygenase (amoA), hydroxylamine oxidoreductase (hao), and nitrogenase (nifH) was analyzed by blastx against curated reference databases. In addition, Polymerase chain reaction (PCR)-based amplification was performed on the hzsA gene of anammox bacteria. Our results reveal high genomic potential for full denitrification to N2, but minor importance of anaerobic ammonium oxidation and dissimilatory nitrite reduction to ammonium. Genomic potential for aerobic ammonia oxidation was dominated by Thaumarchaeota. A higher diversity of anammox bacteria was detected in metagenomes than with PCR-based technique. The results reveal the importance of various N-cycle driving processes and highlight the advantage of metagenomics in detection of novel microbial key players.
Subjects
anammox
Bothnian Sea
dentrification
N-cycle
sediment
DDC Class
550: Geowissenschaften
Lizenz
https://creativecommons.org/licenses/by/4.0/
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