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  4. In situ microscopy for online monitoring of enzymatic processes
 
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In situ microscopy for online monitoring of enzymatic processes

Publikationstyp
Journal Article
Date Issued
2011
Sprache
English
Author(s)
Prediger, Andreas  
Bluma, Arne
Höpfner, Tim
Lindner, Patrick  
Beutel, Sascha  
Müller, Jakob J.  
Technische Biokatalyse V-6  
Hilterhaus, Lutz  
Technische Biokatalyse V-6  
Liese, Andreas  orcid-logo
Technische Biokatalyse V-6  
TORE-URI
https://hdl.handle.net/11420/43894
Journal
Chemical engineering & technology  
Volume
34
Issue
5
Start Page
837
End Page
840
Citation
Chemical Engineering & Technology 34 (5): 837-840 (2011)
Publisher DOI
10.1002/ceat.201000563
Scopus ID
2-s2.0-79955028015
Publisher
Wiley-VCH
In situ Microscopy (ISM) is an optical analysis method that allows non-invasive monitoring of processes in real-time. In this article online monitoring of enzyme carriers and the determination of their mechanical stability are presented as new application areas for ISM. As proof of principle the fragmentation of Lewatit VP OC 1600 particles in a stirred tank reactor is tracked. The image algorithm that was used to analyze the acquired image data is described and the results of the stability study are shown. Additional application possibilities of ISM in the area of two-phase systems are demonstrated. In situ microscopy (ISM) is an optical analysis method that allows noninvasive acquisition of image information from running processes. It is demonstrated how this analysis technique can be used to determine the mechanical stability of enzyme carriers. Furthermore, application possibilities of ISM for the characterization of two-phase systems are presented. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Subjects
Enzyme catalysis
Image processing
In situ microscopy
Process monitoring
DDC Class
530: Physics
570: Life Sciences, Biology
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