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The pH-Dependence of the Hydration of 5-Formylcytosine: an Experimental and Theoretical Study
Citation Link: https://doi.org/10.15480/882.15163
Publikationstyp
Journal Article
Date Issued
2022-04-05
Sprache
English
Author(s)
TORE-DOI
Journal
Volume
23
Issue
7
Article Number
e202100651
Citation
ChemBioChem 23 (7): e202100651 (2022)
Publisher DOI
Scopus ID
Publisher
Wiley
5-Formylcytosine is an important nucleobase in epigenetic regulation, whose hydrate form has been implicated in the formation of 5-carboxycytosine as well as oligonucleotide binding events. The hydrate content of 5-formylcytosine and its uracil derivative has now been quantified using a combination of NMR and mass spectroscopic measurements as well as theoretical studies. Small amounts of hydrate can be identified for the protonated form of 5-formylcytosine and for neutral 5-formyluracil. For neutral 5-formylcytosine, however, direct detection of the hydrate was not possible due to its very low abundance. This is in full agreement with theoretical estimates.
Subjects
aldehyde hydrates | computational chemistry | DNA methylation | epigenetics | modified nucleic acids | TET enzymes
DDC Class
541: Physical; Theoretical
572: Biochemistry
610: Medicine, Health
Publication version
publishedVersion
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The pH‐Dependence of the Hydration of 5‐Formylcytosine an Experimental and Theoretical Study.pdf
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2.2 MB
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