Robust biocatalysts - routes to new diversity

Start Page
31
End Page
51
Citation
Applied Biocatalysis: From Fundamental Science to Industrial Applications: 31-51 (2016-08-16)
Publisher DOI
10.1002/9783527677122.ch3
Scopus ID
2-s2.0-85017413656
Publisher
Wiley-VCH
The continuous need for innovative sustainable technologies has led to an increasing interest in biocatalysts that can be applied in various industrial sectors including chemical, pharmaceutical, textile, paper and pulp, food and feed industries as well as for the production of renewable energy. In order to develop a new generation of efficient biocatalysts, there is a great need to discover novel enzymes exhibiting the desired properties for specific applications. Various strategies that are currently employed include activity-based (microbial and enzyme) screening and sequence-based screening (of gene and metagenome libraries). However, further technologies are necessary to overcome the bottleneck between screening and application. Besides the development of efficient expression systems and fermentation technologies, enzymes of interest have to be further optimized. This can be achieved by sophisticated molecular biology and protein engineering approaches including means of synthetic biology, directed evolution, and construction of fusion proteins.
Subjects
Expression systems
Gene fusions
Metagenomics
Microbial hosts
Protein chimeras
Screening
DDC Class
600: Technik