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Purification of high-complexity peptide microarrays by spatially resolved array transfer to gold-coated membranes
Publikationstyp
Journal Article
Date Issued
2013-03-20
Sprache
English
Journal
Volume
25
Issue
11
Start Page
1598
End Page
1602
Citation
Advanced Materials 25 (11): 1598-1602 (2013)
Publisher DOI
Scopus ID
ISSN
09359648
A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm<sup>2</sup> is demonstrated.
Subjects
high-complexity
high-density
laser printers
microchips
peptide microarrays
purification
DDC Class
600: Technology