Purification of high-complexity peptide microarrays by spatially resolved array transfer to gold-coated membranes

A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm<sup>2</sup> is demonstrated.

Content

Subjects

high-complexity

high-density

laser printers

microchips

peptide microarrays

purification

DDC Class

600: Technology