The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host
Citation Link: https://doi.org/10.15480/882.4305
Publikationstyp
Journal Article
Date Issued
2022-04-01
Sprache
English
Institut
TORE-DOI
Journal
Volume
13
Issue
1
Article Number
1735
Citation
Nature Communications 13 (1): 1735 (2022)
Publisher DOI
Scopus ID
PubMed ID
35365607
Publisher
Nature Publishing Group
Micrarchaeota is a distinctive lineage assigned to the DPANN archaea, which includes poorly characterised microorganisms with reduced genomes that likely depend on interactions with hosts for growth and survival. Here, we report the enrichment of a stable co-culture of a member of the Micrarchaeota (Ca. Micrarchaeum harzensis) together with its Thermoplasmatales host (Ca. Scheffleriplasma hospitalis), as well as the isolation of the latter. We show that symbiont-host interactions depend on biofilm formation as evidenced by growth experiments, comparative transcriptomic analyses and electron microscopy. In addition, genomic, metabolomic, extracellular polymeric substances and lipid content analyses indicate that the Micrarchaeon symbiont relies on the acquisition of metabolites from its host. Our study of the cell biology and physiology of a Micrarchaeon and its host adds to our limited knowledge of archaeal symbioses.
DDC Class
570: Biowissenschaften, Biologie
600: Technik
More Funding Information
T.A.M.B. is a recipient of a Sir Henry Dale Fellowship, jointly funded by the Wellcome Trust and the Royal Society (202231/Z/16/Z). A.S. was supported by the European Research Council (ERC STG ASymbEL: 947317), the Swedish Research Council (VR starting grant 2016-03559 to A.S.) and the NWO-I foundation of the Netherlands Organisation for Scientific Research (WISE fellowship). N.D. was supported by the NWO-I foundation of the Netherlands Organisation for Scientific Research (WISE fellowship to A.S.). L.V. was supported by the Soehngen Institute of Anaerobic Microbiology (SIAM) Gravitation grant (024.002.002) of the Netherlands Ministry of Education, Culture and Science (OCW) and the Netherlands Organization for Scientific Research (NWO).We thank Marianne Baas for technical support with the lipid analysis. S.K. and S.G. were partly funded by the German Research Foundation (DFG) (grant number: 252014092).
Publication version
publishedVersion