In situ microscopy for in-line monitoring of the enzymatic hydrolysis of cellulose

Journal
Analytical chemistry  
Volume
85
Issue
17
Start Page
8121
End Page
8126
Citation
Analytical Chemistry 17 (85): 8121-8126 (2013)
Publisher DOI
10.1021/ac4008495
Scopus ID
2-s2.0-84883485152
Publisher
American Chemical Society
A new in-line method for the monitoring of enzymatic hydrolysis of cellulose is described. Using a new in situ microscope prototype, the noninvasive determination of particle size distributions was possible. For the automated analysis of the acquired images, a new processing algorithm called CelluloseAnalyzer was developed. It enabled tracking of the number of particles and moreover allowed monitoring of the proportions of particle size fractions during the course of enzymatic hydrolysis reactions. Using this technique, significant differences between hydrolysis with endoglucanases and cellulase mixtures were observed. Furthermore, the in situ microscopy results were compared with results from off-line measurements with laser diffraction spectroscopy and gel permeation chromatography. © 2013 American Chemical Society.
DDC Class
570: Biowissenschaften, Biologie
More Funding Information
Deutschland, Bundesministerium für Wirtschaft und Technologie, cluster “Biorefinery 2021”