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  4. Evaluation of fixed-bed cultures with immobilized Lactococcus Lactis ssp. Lactis on different scales
 
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Evaluation of fixed-bed cultures with immobilized Lactococcus Lactis ssp. Lactis on different scales

Citation Link: https://doi.org/10.15480/882.2105
Publikationstyp
Journal Article
Date Issued
2017-07-28
Sprache
English
Author(s)
Faschian, Rebecca  
Eren, Ilyas  
Minden, Steven  
Pörtner, Ralf 
Institut
Bioprozess- und Biosystemtechnik V-1  
TORE-DOI
10.15480/882.2105
TORE-URI
http://hdl.handle.net/11420/2181
Journal
The open biotechnology journal  
Volume
11
Start Page
16
End Page
25
Citation
Open Biotechnology Journal (11): 16-25 (2017)
Publisher DOI
10.2174/1874070701711010016
Scopus ID
2-s2.0-85020376300
Publisher
Bentham Open
Fixed-bed processes, where cells are immobilized within macroporous carriers, are a promising alternative to processes with suspended cells. A scale-up concept is presented in order to evaluate the performance as part of process design of fixed-bed processes. Therefore, Lactococcus lactis cultivation in chemostat and batch mode was compared to fixed bed cultures on three different scales, the smallest being the downscaled Multiferm with 10 mL fixed bed units, the second a 100 mL fixed-bed reactor and the third a pilot scale reactor with 1 L fixed bed volume. As expected, the volume specific lactate productivity of all cultivations was dependent on dilution rate. In suspension chemostat culture a maximum of 2.3 g · L-1·h-1was reached. Due to cell retention in the fixed-beds, productivity increased up to 8.29 g·L-1·h-1at a dilution rate of D = 1.16 h-1(corresponding to 2.4 µmax) on pilot scale. For all fixed bed cultures a common spline was obtained indicating a good scale-up performance.
Subjects
fixed-bed reactor
immobilization
lactococcus lactis
scale-up
DDC Class
500: Naturwissenschaften
570: Biowissenschaften, Biologie
More Funding Information
Federal Ministry of Education and Research (BMBF) grant No. 0.31A124A
Lizenz
https://creativecommons.org/licenses/by/4.0/
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