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Activity and stability of immobilised soybean lipoxygenase-1 in aqueous and supercritical carbon dioxide media
Publikationstyp
Journal Article
Date Issued
2000-11
Sprache
English
Author(s)
Biokatalyse II (V-6H)
Biokatalyse II (V-6H)
Kasche, Volker
Biokatalyse (V-6H)
Journal
Volume
22
Issue
22
Start Page
1815
End Page
1821
Citation
Biotechnology Letters 22 (22): 1815-1821 (2000-11)
Publisher DOI
Scopus ID
Publisher
Kluwer Academic Press
The activity of immobilised soybean lipoxygenase-1 (LOX-1) was studied in aqueous and supercritical carbon dioxide (SCCO2) media for the production of 13S-hydroperoxyoctadecadenoic acid (13S-HPODE). In SCCO2, it was optimal at 33 °C and 25 MPa. A higher space-time yield of 5.7 x 10-3 Ms-1 mg-1 LOX-1 for 13S-HPODE was obtained in SCCO2 compared to only 5 x 10-5 Ms-1 mg-1 LOX-1 in aqueous medium. The stability of immobilised LOX-1 was only significantly affected by the pressurisation and depressurisation steps during reactions in SCCO2.
Subjects
Immobilised soybean lipoxygenase-1
Supercritical carbon dioxide
DDC Class
570: Life Sciences, Biology