Activity and stability of immobilised soybean lipoxygenase-1 in aqueous and supercritical carbon dioxide media

Publikationstyp
Journal Article
Publikationsdatum
2000-11
Sprache
English
Author
Chikere, Anthony Chibuike 
Biokatalyse II (V-6H)
Galunsky, Boris 
Biokatalyse II (V-6H)
Overmeyer, André
Arbeitsbereich Verfahrenstechnik II, Thermische Verfahrenstechnik 6-03 (H) 
Brunner, Gerd 
Arbeitsbereich Verfahrenstechnik II, Thermische Verfahrenstechnik 6-03 (H) 
Kasche, Volker
Biokatalyse (V-6H)
TORE-URI
https://hdl.handle.net/11420/48460
Enthalten in
Biotechnology letters 
Volume
22
Issue
22
Start Page
1815
End Page
1821
Citation
Biotechnology Letters 22 (22): 1815-1821 (2000-11)
Publisher DOI
10.1023/A:1005626806321
Scopus ID
2-s2.0-0033663426
Publisher
Kluwer Academic Press
The activity of immobilised soybean lipoxygenase-1 (LOX-1) was studied in aqueous and supercritical carbon dioxide (SCCO2) media for the production of 13S-hydroperoxyoctadecadenoic acid (13S-HPODE). In SCCO2, it was optimal at 33 °C and 25 MPa. A higher space-time yield of 5.7 x 10-3 Ms-1 mg-1 LOX-1 for 13S-HPODE was obtained in SCCO2 compared to only 5 x 10-5 Ms-1 mg-1 LOX-1 in aqueous medium. The stability of immobilised LOX-1 was only significantly affected by the pressurisation and depressurisation steps during reactions in SCCO2.
Schlagworte
Immobilised soybean lipoxygenase-1
Supercritical carbon dioxide
DDC Class
570: Life Sciences, Biology