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Novel synthetic co-culture of Acetobacterium woodii and Clostridium drakei using CO₂ and in situ generated H₂ for the production of caproic acid via lactic acid
Citation Link: https://doi.org/10.15480/882.5030
Publikationstyp
Journal Article
Date Issued
2023-01
Sprache
English
Institut
TORE-DOI
Journal
Volume
23
Issue
1
Article Number
e2100169
Citation
Engineering in Life Sciences 23 (1): e2100169 (2023-01)
Publisher DOI
Scopus ID
Publisher
Wiley-VCH
Acetobacterium woodii is known to produce mainly acetate from CO₂ and H₂, but the production of higher value chemicals is desired for the bioeconomy. Using chain-elongating bacteria, synthetic co-cultures have the potential to produce longer-chained products such as caproic acid. In this study, we present first results for a successful autotrophic co-cultivation of A. woodii mutants and a Clostridium drakei wild-type strain in a stirred-tank bioreactor for the production of caproic acid from CO₂ and H₂ via the intermediate lactic acid. For autotrophic lactate production, a recombinant A. woodii strain with a deleted Lct-dehydrogenase complex, which is encoded by the lctBCD genes, and an inserted D-lactate dehydrogenase (LdhD) originating from Leuconostoc mesenteroides, was used. Hydrogen for the process was supplied using an All-in-One electrode for in situ water electrolysis. Lactate concentrations as high as 0.5 g L–1 were achieved with the AiO-electrode, whereas 8.1 g L–1 lactate were produced with direct H₂ sparging in a stirred-tank bioreactor. Hydrogen limitation was identified in the AiO process. However, with cathode surface area enlargement or numbering-up of the electrode and on-demand hydrogen generation, this process has great potential for a true carbon-negative production of value chemicals from CO₂.
Subjects
bioelectrochemical system
carbon fixation
cell–cell interaction
constraint-based modeling
in situ electrolysis
DDC Class
600: Technik
Publication version
publishedVersion
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Engineering in Life Sciences - 2022 - Herzog - Novel synthetic co‐culture of Acetobacterium woodii and Clostridium drakei.pdf
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