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Corroboration of mechanobiological simulations of tissue differentiation in an in vivo bone chamber using a lattice-modeling approach
Publikationstyp
Journal Article
Date Issued
2009-12
Sprache
English
Author(s)
Journal
Volume
27
Issue
12
Start Page
1659
End Page
1666
Citation
Journal of Orthopaedic Research 27 (12): 1659-1666 (2009)
Publisher DOI
Scopus ID
Publisher
Wiley
It is well established that the mechanical environment modulates tissue differentiation, and a number of mechanoregulatory theories for describing the process have been proposed. In this study, simulations of an in vivo bone chamber experiment were performed that allowed direct comparison with experimental data. A mechanoregulation theory for mesenchymal stem cell differentiation based on a combination of fluid flow and shear strain (computed using finite element analysis) was implemented to predict tissue differentiation inside mechanically controlled bone chambers inserted into rat tibae. To simulate cell activity, a lattice approach with stochastic cell migration, proliferation, and selected differentiation was adopted; because of its stochastic nature, each run of the simulation gave a somewhat different result. Simulations predicted the load-dependency of the tissue differentiation inside the chamber and a qualitative agreement with histological data; however, the full variability found between specimens in the experiment could not be predicted by the mechanoregulation algorithm. This result raises the question whether tissue differentiation predictions can be linked to genetic variability in animal populations. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
Subjects
Bone chamber
FE-model
Lattice approach
Mechanobiology
Tissue differentiation
DDC Class
610: Medicine, Health