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Relationship between nanoscale mineral properties and calcein labeling in mineralizing bone surfaces
Publikationstyp
Journal Article
Date Issued
2014
Sprache
English
Author(s)
Kerschnitzki, Michael
Hoerth, Rebecca
Burghammer, Manfred
Montero, Cédric
Wagermaier, Wolfgang
Journal
Volume
55
Issue
SUPPL. 1
Start Page
15
End Page
17
Citation
Connective Tissue Research 55: 15-17 (2014)
Publisher DOI
Scopus ID
Publisher
Taylor & Francis
Bone's mineral properties, such as particle thickness and degree of alignment have been associated with bone quality. Bone formation, remodeling, aging of the tissue and mineral homeostasis influence mineral particle properties leading to specific patterns across bone. Scanning small angle X-ray scattering (sSAXS) with synchrotron radiation is a powerful tool, which allows us to study bone's nanoscale mineral properties in a position-resolved way. We used sSAXS, fluorescence light microscopy and backscattered electron (BSE) imaging to study bone's mineral properties at the tibial midshaft of in vivo-loaded mice. By combining these techniques, we could detect local changes in mineral properties. Regions labeled with calcein fluorochrome have lower mean mineral thickness and degree of mineral alignment. We also observed thinner and less aligned mineral particles near blood vessels. We conclude that mineral properties (i) are altered by fluorochrome labeling and (ii) depend on the proximity to blood vessels. © 2014 Informa Healthcare USA, Inc.
Subjects
Bone mineral
Calcein
Mice
Synchrotron sSAXS
DDC Class
610: Medicine, Health