Crystal structures of two tetrameric β-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora

Carbonic anhydrases (CAs) are metalloenzymes catalyzing the reversible hydration of carbon dioxide to bicarbonate (hydrogen carbonate) and protons. CAs have been identified in archaea, bacteria and eukaryotes and can be classified into five groups (α, β, γ, δ, ζ) that are unrelated in sequence and structure. The fungal β-class has only recently attracted attention. In the present study, we investigated the structure and function of the plant-like β-CA proteins CAS1 and CAS2 from the filamentous ascomycete Sordaria macrospora. We demonstrated that both proteins can substitute for the Saccharomyces cerevisiae β-CA Nce103 and exhibit an in vitro CO2 hydration activity (kcat/Km of CAS1: 1.30 × 10 6 m-1·s-1; CAS2: 1.21 × 10 6m-1·s-1). To further investigate the structural properties of CAS1 and CAS2, we determined their crystal structures to a resolution of 2.7 Å and 1.8 Å, respectively. The oligomeric state of both proteins is tetrameric. With the exception of the active site composition, no further major differences have been found. In both enzymes, the Zn2+ -ion is tetrahedrally coordinated; in CAS1 by Cys45, His101 and Cys104 and a water molecule and in CAS2 by the side chains of four residues (Cys56, His112, Cys115 and Asp58). Both CAs are only weakly inhibited by anions, making them good candidates for industrial applications. Structured digital abstract CAS1 and CAS2 bind by x-ray crystallography (View interaction) Database Structural data have been deposited in the Protein Data Bank database under accession numbers 4O1J for CAS1 and 4O1K for CAS2. In this manuscript we determined the crystal structures and the in vitro CO2 hydration activities of the plant-type β-carbonic anhydrases CAS1 and CAS2 of the fungus Sordaria macrospora. The oligomeric state of both proteins is tetrameric. Both CAs are weakly only inhibited by anions making them good candidates for industrial applications. © 2014 FEBS.

Schlagworte

carbon dioxide

crystal structure

enzyme inhibition

Sordaria macrospora

β-class carbonic anhydrase

DDC Class

570: Biowissenschaften, Biologie

Funding Organisations

Deutsche Forschungsgemeinschaft (DFG)

More Funding Information

Deutsche Forschungsgemeinschaft. Grant Number: PO523/5-1
Göttingen Graduate School for Neurosciences
Biophysics und Molecular Biosciences. Grant Numbers: GSC 226/1, GSC 226/2

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Crystal structures of two tetrameric β-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora