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Megahertz serial crystallography
Citation Link: https://doi.org/10.15480/882.2175
Publikationstyp
Journal Article
Date Issued
2018-10-02
Sprache
English
Author(s)
Institut
TORE-DOI
TORE-URI
Journal
Volume
9
Issue
1
Article Number
4025
Citation
Nature Communications 1 (9): 4025 (2018-10-02)
Publisher DOI
Scopus ID
Publisher
Nature Publishing Group UK
The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
DDC Class
600: Technik
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