Erratum to: Protein design and engineering of a de novo pathway for microbial production of 1,3-propanediol from glucose

Publikationstyp
Text
Date Issued
2016-10-04
Sprache
English
Author(s)
Chen, Zhen  
Geng, Feng  
Zeng, An-Ping  orcid-logo
Institut
Bioprozess- und Biosystemtechnik V-1  
TORE-URI
http://hdl.handle.net/11420/5401
Journal
Biotechnology journal  
Start Page
1368
End Page
1368
Citation
Biotechnology Journal 10 (11): 1368-1368 (2016-10-04)
Publisher DOI
10.1002/biot.201670103
Scopus ID
2-s2.0-84989821558
Publisher
Wiley-VCH
The protocol for the activity array of the enzyme glutamate dehydrogenase (GDH) used in our previous publication (doi: 10.1002/biot.201400235) should be corrected as follows: Purified proteins were collected in solutions without supplementation of dithiothreitol in order to avoid its possible influence on the absorbance at 340 nm when measuring the enzymatic activity, especially when homoserine was used as the substrate. With the new protocol, the specific activity of the wild type GDH towards homoserine was determined as 0.18±0.04 mU/mg protein and the specific activity of the mutant K92V/T195S towards homoserine was 3.47±0.01 mU/mg protein. The kinetic parameters of GDH listed in Fig. 2B should also be corrected due to a mistake of units used in calculation of the Vmax.
DDC Class
620: Ingenieurwissenschaften