Impact of anaerobiosis strategy and bioreactor geometry on the biochemical response of Clostridium butyricum VPI 1718 during 1,3-propanediol fermentation
The impact of anaerobiosis strategy on 1,3-propanediol production during cultivation of Clostridium butyricum VPI 1718 in different size bioreactors was studied. In batch trials with N 2 gas infusion, the fermentation was successfully accomplished, regardless of initial glycerol concentration imposed and bioreactor geometry. However, in the absence of N 2 continual sparging, significant variations concerning the biochemical response of the strain were observed. Specifically, at 1-L bioreactor, the absence of N 2 infusion at high initial glycerol concentration induced lactate dehydrogenase activity and thus lactic acid synthesis, probably due to partial blockage of phosphoroclastic reaction caused by insufficient self-generated anaerobiosis environment. During fed-batch cultivation with continual N 2 sparging, the strain produced ∼71gL -1 of 1,3-propanediol, whereas under self-generated anaerobiosis, 1,3-propanediol pathway was evidently restricted, as only 30.5gL -1 of 1,3-propanediol were finally produced. Apparently, N 2 infusion strategy paired with bioreactor geometry can alter the biochemical behavior of the particular strain. © 2011 Elsevier Ltd.
570: Biowissenschaften, Biologie
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Financial support has been kindly provided by the EU (FP7 Program ‘‘PROPANERGY – Integrated bioconversion of glycerine into value-added products and biogas at pilot plant scale’’, Grant number 212671).