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  4. From an extremophilic community to an electroautotrophic production strain: identifying a novel Knallgas bacterium as cathodic biofilm biocatalyst
 
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From an extremophilic community to an electroautotrophic production strain: identifying a novel Knallgas bacterium as cathodic biofilm biocatalyst

Publikationstyp
Journal Article
Date Issued
2020-05-01
Sprache
English
Author(s)
Reiner, Johannes Eberhard  
Geiger, Katharina  
Hackbarth, Max  
Fink, Marielle  
Lapp, Christian 
Jung, Tobias  
Dötsch, Andreas  
Hügler, Michael  
Wagner, Michael  
Hille-Reichel, Andrea  
Wilcke, Wolfgang  
Kerzenmacher, Sven  
Horn, Harald  
Gescher, Johannes 
TORE-URI
http://hdl.handle.net/11420/10220
Journal
ISME journal  
Volume
14
Issue
5
Start Page
1125
End Page
1140
Citation
ISME Journal 14 (5): 1125-1140 (2020-05-01)
Publisher DOI
10.1038/s41396-020-0595-5
Scopus ID
2-s2.0-85078665427
PubMed ID
31996786
Coupling microbial electrosynthesis to renewable energy sources can provide a promising future technology for carbon dioxide conversion. However, this technology suffers from a limited number of suitable biocatalysts, resulting in a narrow product range. Here, we present the characterization of the first thermoacidophilic electroautotrophic community using chronoamperometric, metagenomic, and 13C-labeling analyses. The cathodic biofilm showed current consumption of up to −80 µA cm−2 over a period of 90 days (−350 mV vs. SHE). Metagenomic analyses identified members of the genera Moorella, Desulfofundulus, Thermodesulfitimonas, Sulfolobus, and Acidianus as potential primary producers of the biofilm, potentially thriving via an interspecies sulfur cycle. Hydrogenases seem to be key for cathodic electron uptake. An isolation campaign led to a pure culture of a Knallgas bacterium from this community. Growth of this organism on cathodes led to increasing reductive currents over time. Transcriptomic analyses revealed a distinct gene expression profile of cells grown at a cathode. Moreover, pressurizable flow cells combined with optical coherence tomography allowed an in situ observation of cathodic biofilm growth. Autotrophic growth was confirmed via isotope analysis. As a natural polyhydroxybutyrate (PHB) producer, this novel species, Kyrpidia spormannii, coupled the production of PHB to CO2 fixation on cathode surfaces.
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