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Multi-fixed-bed bioreactor system applied for bioprocess development of immobilized lactic acid bacteria
Citation Link: https://doi.org/10.15480/882.1998
Publikationstyp
Journal Article
Date Issued
2016-02-19
Sprache
English
Author(s)
Institut
TORE-DOI
Journal
Volume
10
Issue
1
Start Page
1
End Page
9
Citation
Open Biotechnology Journal 1 (10): 1-9 (2016-02-01)
Publisher DOI
Scopus ID
Publisher
Bentham Open
The multi-fixed-bed bioreactor “Multiferm” provides a downscaled system for the development of fixed-bed processes with immobilized microorganisms or cells. It consists of twelve single fixed-bed units that can be operated independently. Different bacterial strains, carriers used for immobilization, media or flow rates can be studied in one compact experimental set-up. Experiments can be run in parallel to allow for determination of experimental errors. In these studies several continuous cultivations were carried out with Lactobacillus delbrueckii ssp. bulgaricus using different dilution rates and culture durations as proof-ofconcept for the Multiferm. Parameters relevant for process design such as reproducibility of simultaneously performed cultures, optimal dilution rate and long-term performance were determined by means of volume specific lactate and biomass productivity. First indications on a reduced growth rate of the cells due to immobilization were found.
Subjects
Cell density
continuous cultivation
dilution rate
experimental error
fixed-bed
lactic acid bacteria
metabolic activity
process development
scale-down
DDC Class
570: Biowissenschaften, Biologie
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