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  4. Proinflammatory and osteoclastogenic effects of beta-tricalciumphosphate and hydroxyapatite particles on human mononuclear cells in vitro
 
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Proinflammatory and osteoclastogenic effects of beta-tricalciumphosphate and hydroxyapatite particles on human mononuclear cells in vitro

Publikationstyp
Journal Article
Date Issued
2009-10-01
Sprache
English
Author(s)
Lange, Tobias  
Schilling, Arndt F.  
Biomechanik M-3  
Peters, Fabian  
Haag, Friedrich
Morlock, Michael  
Biomechanik M-3  
Rueger, Johannes M.  
Amling, Michael  
TORE-URI
https://hdl.handle.net/11420/46532
Journal
Biomaterials  
Volume
30
Issue
29
Start Page
5312
End Page
5318
Citation
Biomaterials 30 (29): 5312–5318 (2009)
Publisher DOI
10.1016/j.biomaterials.2009.06.023
Scopus ID
2-s2.0-68549104032
Publisher
Elsevier
Particulate wear debris can activate defence cells and osteoclasts at the bone-implant interface possibly leading to bone resorption and implant failure. Cellular responses and inflammatory effects have been reported for particulate hydroxyapatite (HA). However, the immunological effects of particulate beta-tricalciumphosphate (beta-TCP) have not been studied and the question of whether beta-TCP is more biocompatible in this regard as is HA remains to be determined. Therefore the present work investigates effects of endotoxin-free HA and beta-TCP particles of the same size (d50 = 1 μm) and dose (SAR 10:1) on human peripheral blood mononuclear cells in vitro. The production of proinflammatory cytokines (TNF-alpha, IL-1beta, IL-8) and cytokines connected to osteoclast and dendritic cell differentiation (OPG, RANKL, M-CSF, GM-CSF) was determined by ELISA. After 6 and 18 h of incubation HA and beta-TCP caused a quite similar induction of TNF-alpha, IL-1beta and IL-8. Effects of particles on the production of M-CSF and OPG were not detectable. However, in sharp contrast to HA, beta-TCP caused less induction of GM-CSF and not any of RANKL, both known for promoting dendritic cells and osteoclastogenesis respectively. Therefore these in vitro data suggest that wear debris of beta-TCP poses lesser risk of the detrimental effects of osteoclast induction known from HA.
Subjects
Beta-TCP
Dendritic cells
Human PBMC
Hydroxyapatite
Inflammation
Osteoclastogenesis
DDC Class
610: Medicine, Health
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