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A multi-enzyme cascade for the production of High-Value Aromatic Compounds
Citation Link: https://doi.org/10.15480/882.3028
Publikationstyp
Journal Article
Publikationsdatum
2020-10-20
Sprache
English
TORE-URI
Enthalten in
Volume
10
Start Page
1
End Page
13
Citation
Catalysts 10 (10): 1216 (2020)
Publisher DOI
Scopus ID
Publisher
Multidisciplinary Digital Publishing Institute
Cascade reactions are the basis of life in nature and are adapted to research and industry in an increasing manner. The focus of this study is the production of the high-value aromatic ester cinnamyl cinnamate, which can be applied in flavors and fragrances. A three-enzyme cascade was established to realize the synthesis, starting from the corresponding aldehyde with in situ cofactor regeneration in a two-phase system. After characterization of the enzymes, a screening with different organic solvents was carried out, whereby xylene was found to be the most suitable solvent for the second phase. The reaction stability of the formate dehydrogenase (FDH) from <i>Candida boidinii</i> is the limiting step during cofactor regeneration. However, the applied enzyme cascade showed an overall yield of 54%. After successful application on lab scale, the limitation by the FDH was overcome by immobilization of the enzymes and an optimized downstream process, transferring the cascade into a miniplant. The upscaling resulted in an increased yield for the esterification, as well as overall yields of 37%.
DDC Class
620: Ingenieurwissenschaften
More Funding Information
Deutsche Forschungsgemeinschaft: BU3409/1-1 and WA 3957/1-1
Publication version
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