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  4. Inteins, valuable genetic elements in molecular biology and biotechnology
 
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Inteins, valuable genetic elements in molecular biology and biotechnology

Citation Link: https://doi.org/10.15480/882.1922
Publikationstyp
Review Article
Date Issued
2010-05-07
Sprache
English
Author(s)
Elleuche, Skander  
Pöggeler, Stefanie  
Institut
Technische Mikrobiologie V-7  
TORE-DOI
10.15480/882.1922
TORE-URI
http://tubdok.tub.tuhh.de/handle/11420/1925
Journal
Applied microbiology and biotechnology  
Volume
87
Issue
2
Start Page
479
End Page
489
Citation
Applied microbiology and biotechnology 2 (87): 479-489 (2010-06)
Publisher DOI
10.1007/s00253-010-2628-x
Scopus ID
2-s2.0-77953043452
Publisher
Springer
Inteins are internal protein elements that self-excise from their host protein and catalyze ligation of the flanking sequences (exteins) with a peptide bond. They are found in organisms in all three domains of life, and in viral proteins. Intein excision is a posttranslational process that does not require auxiliary enzymes or cofactors. This self-excision process is called protein splicing, by analogy to the splicing of RNA introns from pre-mRNA. Protein splicing involves only four intramolecular reactions, and a small number of key catalytic residues in the intein and exteins. Protein-splicing can also occur in trans. In this case, the intein is separated into N- and C-terminal domains, which are synthesized as separate components, each joined to an extein. The intein domains reassemble and link the joined exteins into a single functional protein. Understanding the cis- and trans-protein splicing mechanisms led to the development of intein-mediated protein-engineering applications, such as protein purification, ligation, cyclization, and selenoprotein production. This review summarizes the catalytic activities and structures of inteins, and focuses on the advantages of some recent intein applications in molecular biology and biotechnology.
Subjects
intein
protein splicing
protein purification
cyclic proteins
selenoproteins
DDC Class
570: Biowissenschaften, Biologie
Lizenz
https://creativecommons.org/licenses/by-nc-nd/3.0/
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